Fig. 7.
![Fig. 7. Intracellular localization of VE-cadherin in VECD-1–treated ECs. / (A-F) The addition of 20 μg/mL VECD-1 disrupted EC-EC contact, thereby allowing ECs to scatter. VE-cadherin was translocated from cell surfaces to the intracellular cytoplasm (panel E, arrow). Internalized VE-cadherin colocalized with γ-catenin (panel A, arrow) (γ-catenin–VE-cadherin [VECD-1]; panels A-B). (D-F) In contrast, ZO1 remains at the cell-cell junctions (ZO1–secondary Ab; panels D-E). Panels C and F are merged images. Scale bar, 20 μm.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/4/10.1182_blood-2002-05-1329/3/h80433817007.jpeg?Expires=1724242803&Signature=Lf77TdvkYqBaOWzC2GCjn-nElb2bhE~2fJw35PmCEt~jfXkdHpjm3CmPqwVpuJLa60cMouANlPHxuBshgdU5Nv1gh3WnJWUK2oAWjw1EVydKp-BgN-2jWPdpVze8I3ubVfySxkP-jrTT5AhkHPvJx88yAQDfVpW11AKBKuKmkMrI072GhnCuhdc8FX5Wmnka9CWFM59lcVE8Lr3UTzg4F5pPrfpVlNtYoHz7jNLmLXXbi~7Cc31mSSOoiN7b8nLksqlrbjAM-daqMIXpEIqnVD-4vw00XykQECGRP374XiqxF2DAzjjW7jHAAIXBgHKF36YxvghpU1JoeSi4B01t0Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Intracellular localization of VE-cadherin in VECD-1–treated ECs.
(A-F) The addition of 20 μg/mL VECD-1 disrupted EC-EC contact, thereby allowing ECs to scatter. VE-cadherin was translocated from cell surfaces to the intracellular cytoplasm (panel E, arrow). Internalized VE-cadherin colocalized with γ-catenin (panel A, arrow) (γ-catenin–VE-cadherin [VECD-1]; panels A-B). (D-F) In contrast, ZO1 remains at the cell-cell junctions (ZO1–secondary Ab; panels D-E). Panels C and F are merged images. Scale bar, 20 μm.
