Inhibition of STAT3 phosphorylation by AG490 in MM cell lines.

After starvation for 16 hours, cells were resuspended in complete culture medium with (+) or without (−) 50 μM AG490 and incubated for 1 hour and 24 hours. Cell lysates were analyzed by Western blot with anti–phospho-STAT3 antibody, and subsequently with anti-STAT3 antibody.