AG490 induces apoptosis in cell lines with hypermethylation-associated silencing of SOCS-1.

Cells were incubated with or without 50 μM AG490 for 96 hours before annexin V/PI staining and analysis by flow cytometry. Cells that are annexin V⁺ and PI⁻ (lower right quadrants) represent early apoptotic cells, and cells that are annexin V⁺ and PI⁺ (upper right quadrants) represent late apoptotic and/or necrotic cells. The percentages of cells in the upper and lower right quadrants are indicated.