SOCS-1 MSP analysis of hematopoietic cell lines, normal peripheral blood (PB), and nonmalignant bone marrow (BM).

Lane U indicates amplified product with primers recognizing unmethylated SOCS-1 sequence. Lane M indicates amplified product with primers recognizing methylated SOCS-1 sequence. The cell lines KG1a, U266, and XG1 show aberrant hypermethylation of the SOCS-1 CpG island. In vitro–methylated DNA (IVD) and water served as controls.