BM cells cultured with M-CSF express genes for components of Notch signaling and RANK.

Freshly prepared BM cells were cultured with M-CSF (50 ng/mL). On days 2 and 4 of culture, dish-adherent and -nonadherent cells were harvested and the gene expression for Notch receptors and their ligands, and RANK (Tnfrsf11a) was examined by RT-PCR analyses. The expression of these genes was enhanced by culturing with M-CSF.