Fig. 3.
Fig. 3. Induction of Hes1 gene expression by immobilized Delta-1–FL. / Total RNAs were prepared from dish-adherent BM cells cultured in the presence of immobilized Delta-1–FL or BAP-FL, or soluble Delta-1–FL, plus M-CSF (50 ng/mL) for 4 days. Serial dilutions (1:1, 1:9, 1:81, 1:729) of cDNAs were subjected to PCR amplification specific for theHes1 and Gapd genes.

Induction of Hes1 gene expression by immobilized Delta-1–FL.

Total RNAs were prepared from dish-adherent BM cells cultured in the presence of immobilized Delta-1–FL or BAP-FL, or soluble Delta-1–FL, plus M-CSF (50 ng/mL) for 4 days. Serial dilutions (1:1, 1:9, 1:81, 1:729) of cDNAs were subjected to PCR amplification specific for theHes1 and Gapd genes.

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