C7-TY, ST2, and BM cells express genes for components of Notch signaling, and immobilized Delta-1–FL directly inhibits osteoclastogenesis of C7-TY cells.

(A) RT-PCR analyses of genes for components of Notch signaling were performed by using cDNAs from C7-TY, ST2, and BM cells. (B) C7-TY cells cultured with RANKL (25 ng/mL) on dishes coated with Delta-1–FL or BAP-FL, or in the presence of soluble Delta-1–FL (1 μg/mL) or BAP-FL (1 μg/mL). After 6 days of culturing, the number of TRAP⁺-MNCs was counted. Bars indicate means ± SDs of triplicate cultures. *Indicates significantly different from the corresponding cultures in dishes coated with BAP-FL (P < .005).