Inhibition of MAP kinase phosphorylation and IL-8 secretion by LXA4.

(A) Neutrophils were stimulated with 1 μM SAA or 5 μM LXA4, or with SAA and LXA4. After 15 minutes, samples were collected for detection of ERK1/2 and p38 phosphorylation by antiphospho antibodies against these kinases. The results are from a representative experiment of 3 separate experiments. (B) Neutrophils were stimulated with SAA, in the presence or absence of LXA4 as indicated. After 4 hours, the secreted IL-8 was measured by ELISA. The induction of IL-8 in the absence and presence of LXA4 was compared. Data are shown as mean ± SEM of 3 independent experiments, each performed in duplicate.