Cytotoxicity of autologous T cells primed with IL-7LN-transduced leukemic DCs or with control LXLN-transduced DCs against autologous AML blasts.

Effector cells were autologous T cells cultured with irradiated IL-7-modified leukemic DCs or control LXLN leukemic DCs for 14 days. Autologous AML blasts were used as target cells. Panel A represents the cytotoxicity of triplicate cultures (means ± SDs) obtained at a different effector-target ratio in patient no. 2. The significance between the IL-7LN (•) and control group (▵, LXLN) at different E/T ratio is indicated as: *P < .03 and **P < .005. Panel B shows the phenotype of the effector T cells used in the cytotoxic assay. Cells were stained with CD8-FITC and CD4-PE mAbs and analyzed by flow cytometry. Makers were set according to an isotype-control mAb. The number in each box represents the percent of positive cells.