Fig. 5.
Cytokine-transduced leukemia cells increase IL-2 and γIFN production in T cells.
Leukemia cells transduced with the IL-7LN or control (LXLN) vector were differentiated in a serum-free cytokine cocktail for one week. Subsequently, 4 to 5 × 104 unsorted or sorted cells were cultured alone or with 105 T cells. After 48 to 72 hours supernatants were harvested and measured for IL-2 and γIFN production using specific ELISA. Panel A shows IL-2 production in 3 different patients using unsorted IL-7LN and LXLN populations. Panel B shows (in 2 different scales) IL-2 and γIFN production by sorted IL-7LN and LXLN-transduced cells from patient no. 2. Controls included in the study are indicated.
