Proliferation of allogeneic T cells in response to transduced and sorted populations.

Leukemia cells from patient no. 3 (A) and patient no. 5 (B) were transduced with both IL-7LN and control vector (LXLN). Cell-sorting selection was based on LNGFr expression using a specific mAb. Sorting subsets IL-7LN⁺, LXLN⁺, and LXLN⁻were replated in a serum-free differentiation cytokine cocktail for an additional 7 days and used as stimulators at different stimulator-responder ratios. Panel B also includes fresh leukemia cells as stimulators. Allo-MLR was performed with 5 × 10⁴ T cells as responders. Proliferation was measured 5 days after stimulation by ³H-thymidine incorporation (cpm). Indicated values represent the mean of triplicates.