Immunophenotype of IL-7LN-transduced leukemic DCs.

Leukemic cells from patient no. 5 were cultured in a cytokine containing serum-free medium (see “Patients, materials, and methods”) for 48 hours prior to 3 rounds of retroviral transduction with the IL-7LN vector. On day 7, cells were replated in a differentiation cytokine cocktail (see “Patients, materials, and methods”) for an additional 8 days. Double stainings with the LNGFr and CD86, CD80, CD83, HLA-DR, HLA-DQ, CD1a, CD40, CD54, and CD58 mAbs were performed at indicated time point. Viable cells were gated based on forward scatter and side scatter (FSC-SSC) features. Markers were set according to an isotype-matched control mAb. Fluorescence intensities are displayed in logarithmic scale. The numbers represent the percentages of positive cells.