Neutrophilic infiltration at the footpad injection site of CD95L-DC injection is IL-1 dependent.
C57BL/6 WT (A-C) and IL-1 R−/− (D-F) mice were injected subcutaneously with 3 × 105 bm12-lpr control (A, D) or CD95L-DCs (B-F). Footpad skin sections were realized 5 days later and stained with hematoxylin and eosin. Panels A and D show the histology of a conserved skin structure with negligible cell infiltration (original magnification × 100); panel B, an altered skin structure with dermis thickening and strong inflammation extended until the muscle cells layer (original magnification × 100); and panel C, a massive neutrophil recruitment (arrow) into the dermis (original magnification × 1000). Panel E shows a conserved skin histology with much less inflammatory cells (original magnification × 100), and panel F, mononuclear cells plus eosinophils (arrow) infiltrate located only into the dermis (original magnification × 1000). Representative sections from 3 to 6 individual mice were selected.
