CD95L-transduced DCs are cytotoxic and down-regulate allogeneic MLC in vitro.
(A) mCD95-transfected P815 cells were incubated with CD95L-DCs (●) or control DCs (○). Data are representative of 15 experiments. (B) CD95-P815 cells were cocultured with CD95L-DCs (right panel) or CD95L-transfected PhoenixECO cells (left panel) at an effector/target cell ratio of 2.5:1, in the presence or not of mFas-hFc. Percentages of lysis are representative of 2 experiments. (C) Triplicate culture of 2 × 105 bm12 LN cells and lpr/lpr CD95L (without LPS, ▵; with LPS, ▴) or control DCs (without LPS, ■; with LPS, ▪) was incubated for 3 days. Results are representative of 3 experiments. (D) 106 BALB/c LN cells were seeded with 3 × 105 irradiated C57BL/6 lpr/lpr CD95L or control DCs activated or not with LPS. Supernatants were collected after 72 hours for IFN-γ quantification. Similar data were obtained in 3 experiments.
