IVIG addition stimulates B-lymphocyte differentiation.

Purified human B lymphocytes were cultured with IL-4 and irradiated L4.5 during 14 days with 4 mM glycine and 10 mg/mL BSA (A,C,E) or with 10 mg/mL dialyzed IVIGs (B,D,F). On day 8, cells were collected, washed, and stained for CD19 and IgM (A-B), CD19 and IgG (C-D), or CD19 and intracellular IgG expression (E-F). Both IVIG preparations induced similar patterns during the 14-day culture period.