No cleavage of G-CSF receptor by elastase.

After 7 days of culture with 10 ng/mL G-CSF, GM-CSF, SCF with or without 1 μg/mL elastase, CD34⁺ cells were harvested and stained with anti–CD66b–FITC, anti–G-CSFR–PE antibodies (filled histogram), or appropriate isotype control (open histogram). Peripheral blood neutrophils were incubated overnight with 1 μg/mL elastase and then stained with the same antibodies.