Fig. 2.
Fig. 2. Apoptosis and maturation arrest induced by elastase. / CD34+ cells (5 × 105/mL) were cultured in the presence of 25 ng/mL G-CSF, GM-CSF, and SCF with or without 1 μg/mL elastase. (A) Percentage apoptotic cells determined by morphologic analysis. (B) Maturation index. (C) CD66b+cells day 9. (D) Percentage apoptotic cells determined by annexin V analysis. Data are presented as mean ± SEM of 2 independent experiments. ● indicates control; and ○, elastase.

Apoptosis and maturation arrest induced by elastase.

CD34+ cells (5 × 105/mL) were cultured in the presence of 25 ng/mL G-CSF, GM-CSF, and SCF with or without 1 μg/mL elastase. (A) Percentage apoptotic cells determined by morphologic analysis. (B) Maturation index. (C) CD66b+cells day 9. (D) Percentage apoptotic cells determined by annexin V analysis. Data are presented as mean ± SEM of 2 independent experiments. ● indicates control; and ○, elastase.

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