Differential induction of neutrophil secondary granule genes mediated by C/EBP family members.
(A) NIH 3T3 cells were transiently transfected with 3.0 μg empty expression vector (−), human C/EBPε32, or human C/EBPα. RT-PCR analysis for the genes MCLP, NC, or B9 was performed. A cDNA prepared from 32Dcl3 cells served as a positive control (+). Products were analyzed on 2% agarose gels, Southern blotted, and hybridized with radiolabeled, gene-specific probes (Table 1). The lower panel represents a Western blot of the protein extracted from the organic phase of the TRIzol lysate prepared from the transfected cells. (B) NIH 3T3 cells were transfected with expression vectors for human C/EBPε32, C/EBPε30, C/EBPβ, and C/EBPδ and were analyzed for expression as described above. The lower panel represents a Western blot simultaneously probed for each family member of the protein extracted from the organic phase of the TRIzol lysate prepared from the transfected cells.
