Bone marrow of C/EBPε-deficient mice lacks mRNA expression for the eosinophil granule proteins MBP and EPX.

(A) Northern blot analysis of total RNA (5 μg) from one wild-type (WT) and 2 C/EBPε-null (KO-1 and KO-2) mice. The blot was hybridized sequentially with probes MBP, EPX, and β-actin. (B) RT-PCR analysis of MPO and EPX gene expression in the total bone marrow RNA from one wild-type and 2 C/EBPε-null mice. PCR of cDNAs was performed with primers specific for either MPO or EPX (35 cycles). Products were electrophoresed on a 2% agarose gel, Southern blotted, and hybridized with radiolabeled, gene-specific oligonucleotides (Table 1).