American Society of Hematology

Fig. 1.

$Fig. 1. NP-1 expression on hematopoietic cells. / (A) RT-PCR analysis of NP-1 and VEGF-A expression in various fractions of hematopoietic cells from the fetal liver. High NP-1 expression was detected in CD45+B220+ and CD45+Mac-1+ cells; however, very low NP-1 expression was detected in the fraction of stem cells marked as CD45+CD34+c-kit+ cells. RNA from CD45–PECAM-1+ ECs was used for positive control. (B) Histologic analysis of NP-1 expression on hematopoietic cells in embryo. (i) Transversal section of spinal cord stained with anti–NP-1 antibody. Boxes are viewed in higher power in panels ii and iii. (ii) Round cells in the lumen of a vessel (arrows) and ECs lining the vessel (arrowheads) are positively stained with anti–NP-1 antibody. (iii) Neuronal cells in the DRG are positively stained with anti–NP-1 antibody. (iv) Serial section of panel ii stained with anti-CD45 antibody. Round cells in the vessel as observed in panel ii are positively stained with anti-CD45 antibody. Positively stained cells are visualized as dark blue products. Isotype-matched control Ig for anti-CD45 or anti–NP-1 antibody did not show any staining. Scale bar indicates 50 μm (i,iii); 20 μm (ii,iv).$

NP-1 expression on hematopoietic cells.

(A) RT-PCR analysis of NP-1 and VEGF-A expression in various fractions of hematopoietic cells from the fetal liver. High NP-1 expression was detected in CD45⁺B220⁺ and CD45⁺Mac-1⁺ cells; however, very low NP-1 expression was detected in the fraction of stem cells marked as CD45⁺CD34⁺c-kit⁺ cells. RNA from CD45–PECAM-1⁺ ECs was used for positive control. (B) Histologic analysis of NP-1 expression on hematopoietic cells in embryo. (i) Transversal section of spinal cord stained with anti–NP-1 antibody. Boxes are viewed in higher power in panels ii and iii. (ii) Round cells in the lumen of a vessel (arrows) and ECs lining the vessel (arrowheads) are positively stained with anti–NP-1 antibody. (iii) Neuronal cells in the DRG are positively stained with anti–NP-1 antibody. (iv) Serial section of panel ii stained with anti-CD45 antibody. Round cells in the vessel as observed in panel ii are positively stained with anti-CD45 antibody. Positively stained cells are visualized as dark blue products. Isotype-matched control Ig for anti-CD45 or anti–NP-1 antibody did not show any staining. Scale bar indicates 50 μm (i,iii); 20 μm (ii,iv).

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