Fig. 7.
Real-time RT-PCR analysis of C/EBPα expression.
Total RNA was isolated 4 days after electroporation with 200 nM siRNA and analyzed by real-time RT-PCR as described in “Materials and methods.” The relative C/EBPα mRNA levels in relation to GAPDH mRNA levels are shown. Black bars (▪), no TGFβ1/vitamin D3 treatment; hatched bars (▧), treatment with TGFβ1 and vitamin D3. For siRNA nomenclature and target sites, see Table 1.
