siRNA influence on TGFβ/vitamin D₃–induced CD11b expression.

(A) Graphical presentation of siAM-dependent CD11b expression in Kasumi-1 cells. Black bars (▪), no TGFβ/vitamin D₃treatment; hatched bars (▧), treatment with TGFβ₁/vitamin D₃. (B) Graphical presentation of siAGF1-dependent CD11b expression in Kasumi-1 cells. Black bars (▪), no TGFβ/vitamin D₃ treatment; hatched bars (▧), treatment with TGFβ₁/vitamin D₃. (C) Effect of AML1/MTG8 and AML1 siRNAs on CD11b expression in Kasumi-1 cells. The graph shows the percentage of CD11b-positive Kasumi-1 cells after electroporation with the indicated siRNA followed by addition of TGFβ₁/vitamin D₃. (D) Graphical presentation of siAM-dependent CD11b expression in SKNO-1 cells. Black bars (▪), no TGFβ₁/vitamin D₃ treatment; hatched bars (▧), treatment with TGFβ₁/vitamin D₃. In each experiment, cells were electroporated with 200 nM of the indicated siRNA. For siRNA nomenclature and target sites, see Table 1.