Flow cytometric analysis of the in vitro differentiation of sorted VEGFR2⁺ cells into EC lineage under a chemically defined condition.

Sorted VEGFR2⁺ cells were cultured on type IV collagen–coated dishes under a chemically defined condition in the presence of 30 or 60 ng/mL VEGF. The expression of VE-cadherin and CD34 was analyzed after 2 or 4 days of culture. The numbers indicate the percentage of cells that appeared in each quadrant.