Effect of OSM on murine fetal liver cells from primary cultures.

Fetal hepatic cells derived from 14 dpc fetal liver were cultured for 10 days in long-term culture medium in the absence or presence of 10 ng/mL OSM. Cells were then washed, fixed, and stained with mouse anti–CK-8, –CK-19 (green), and -ASMA (red). Cells were also double-labeled with antibodies to epithelial and hematopoietic cells using goat anti-AFP (red) and rat anti-CD45 (green). Each panel represents a different field. Scale bar = 5 μm.