Phenotypic analysis of AFT024 cells.

(A) AFT024 cells were cultured for 5 days and permeabilized before staining of intracellular components and FACS analysis. Specific stainings are indicated by blackened histogram, and isotype control is shown as open histogram. (B) Western blots for mesenchymal markers: osteopontin (OSP), calponin (calp), ASMA, thrombospondin-1 (TSP), and EDa fibronectin (FN3E2) in AFT024 cells. (C) RT-PCR analysis of the myocyte-enhancer factor (MEF2C) and hepatocyte nuclear factor (HNF3α) in AFT024 cells.