Schematic representation of DNA copy-number alterations in patients with transformed FCL and FCL-derived cell lines.

(A) Chromosome 12: array CGH analysis of 2 transformed samples from patients with FCL (IL114C and IL124B) and 6 cell lines (VAL, Karpas 231, OZ, BEVA, Karpas 353, and SUDHL6). Ninety-one BAC and P1-derived clones included in the array are listed in the columns on the left. In dark green, regions of genomic gain; in bright green, regions of genomic amplification; in red, regions of genomic loss; in white, normal log₂ ratio; gray squares represent noninformative clones. The log₂ ratios for defining genomic imbalances are defined in “Patients, materials, and methods.” The common region of gain was defined between 12p telomere and 12q12, including high-level amplification of clone RP11-29G3 in IL114C (log₂ ratio + SD, +0.96 + 0.059). (B) Chromosome 7q: array CGH analysis of 8 cell lines with gain of chromosome 7q (Karpas 231, OZ, OCI-LY8, DOHH2, SUDHL6, PR1, ROS50, and RL) and one transformed patient (IL 114C). Using CGH to chromosomes, the common region of gain in all these samples allowed the delineation of a common region of gain to 7q11-q31 (data not shown). This region is represented here using array CGH. Fifty-two clones covering 7q11-q31 are shown. The different colors represent the genomic aberrations as defined in Figure4A. The common region of gain was delineated between 7q11.2 and 7q22.1. One clone (CTC-224F8 in 7q21.3) showed high-level amplification in ROS50 (log₂ ratio + SD, +1.13 + 0.022).