ProGP-1–expanded spleen cells produce increased IL-10 and TGFβ and inhibit TNFα production after allogeneic SCT.
Unfractionated spleen cells from control- (■), G-CSF– (▨) or ProGP-1–treated (▪) donors were stimulated in vitro with LPS. IL-10 (A) and TGFβ (B) were determined in 48-hour culture supernatants by ELISA. Results are mean ± SD of triplicate wells and represent one of 3 identical experiments. (C) Animals received transplants of whole control spleen (■, n = 4), G-CSF spleen (▨, n = 4), or ProGP-1 spleen (▪, n = 4) as in Figure 1. Peritoneal macrophages were harvested from animals 7 days after SCT and stimulated with LPS. TNFα was determined in 5-hour culture supernatants by ELISA. Results are normalized to production per 105 macrophages based on CD11b staining. * P < .05 versus control spleen. ND indicates not detected.
