AcSDKP promotes an angiogenic response in the chicken embryo CAM.

AcSDKP, AcS_DDKP, and HARP were diluted in 20 μL distilled water and applied to a 1-cm² area of the CAM on the ninth day of embryo development. The vascular density was estimated 48 hours later using image analysis software. Distilled water, which was used as a solvent, served as an excipient control. AcS_DDKP, a stereoisomer of AcSDKP, was used as a negative peptide control and HARP as a positive control. Data are the mean ± SEM of 3 independent experiments each using 10 to 20 embryonated eggs per data point. **P < .01; ***P < .001.