DNA laddering in PMNs.

DNA extracted from freshly isolated PMNs as well as from PMNs cultured for 6 hours without additions, with 20 ng/mL TNF-α, with 150 μM zVAD-fmk or with the combination of these agents, was separated by agarose gel electrophoresis, and internucleosomal fragmentation (DNA laddering) was assessed. Results are representative of 3 separate experiments.