Fig. 7.
![Fig. 7. Presentation of IYVLVMLVL is TAP independent. / IYV-CTL is a clone specific for peptide no. 11, IYVLVMLVL; RYS-CTL, a clone specific for peptide RYSIFFDYM. HEK293T or T2-A24 cells were transfected with pcDNA3 expressing LMP2 (pcL2), pcDNA3 expressing EBNA3A (pcE3A), pcDNA3 expressing the minimal IYVLVMLVL epitope (pcIYV), or pcDNA3 expressing the minimal RYSIFFDYM epitope (pcRYS), and used as APCs. Plasmids expressing HLA A*2402 were cotransfected into HEK293T cells. T2-A24 cells pulsed with the peptides IYVLVMLVL (IYV) or RYSIFFDYM (RYS) were also used as stimulators. CD8+ T cells (200 [▪]; 100 [░]; 50 [▨]; or 25 [■]) were cultured with the indicated APCs in each well. Each bar represents the average number of spots in duplicate wells.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/4/10.1182_blood-2002-04-1240/3/h80433810007.jpeg?Expires=1722802795&Signature=WCOENbSwp0vi0OhWpfeipgGSzaUjDwo7tLBSh82RRRNjyLbZaO03yFISwfnYD2oAS~BUaudUAL0a2IUNsJNdUY4rkoAX3U03HgZ0eRNQI29rkqsRegOR0aZIe~Oi~1xZ9UQi2ISTrQD7bCDYXYN6n5yoD6INxO7uN2SEp9hkrVbSYl0bEj5SlqOlcm~wkYDQEkH~-I2SKgIczdJFVCMIGvEOGS-kYP~Ucw0wVROm0hWwjA0OxIRM0HbjDpUbnj7I38bMudUa~CMjS-Ku4hYyuN-uH9cdBbgRoJsIFSEQOo3dPtgmoK9jQDPS263lrNQlUrYhTgU009y5Ajrm4IfeSg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Presentation of IYVLVMLVL is TAP independent.
IYV-CTL is a clone specific for peptide no. 11, IYVLVMLVL; RYS-CTL, a clone specific for peptide RYSIFFDYM. HEK293T or T2-A24 cells were transfected with pcDNA3 expressing LMP2 (pcL2), pcDNA3 expressing EBNA3A (pcE3A), pcDNA3 expressing the minimal IYVLVMLVL epitope (pcIYV), or pcDNA3 expressing the minimal RYSIFFDYM epitope (pcRYS), and used as APCs. Plasmids expressing HLA A*2402 were cotransfected into HEK293T cells. T2-A24 cells pulsed with the peptides IYVLVMLVL (IYV) or RYSIFFDYM (RYS) were also used as stimulators. CD8+ T cells (200 [▪]; 100 [░]; 50 [▨]; or 25 [■]) were cultured with the indicated APCs in each well. Each bar represents the average number of spots in duplicate wells.
