Fig. 6.
![Fig. 6. Results of ELISPOT assays using CD8+ T-cell clones. / IYV-CTL is a clone specific for peptide no. 11, IYVLVMLVL; RYS-CTL, a clone specific for peptide RYSIFFDYM. LCL1 and 2 were established from PBMCs of HLA A24–positive donors, and LCL3 and 4 from HLA A24–negative donors. Abbreviations used for describing the stimulators are as follows: IYV, T2-A24 cells pulsed with peptide IYVLVMLVL; RYS, T2-A24 cells pulsed with peptide RYSIFFDYM; vL2, cells infected with vLMP2; vE3A, cells infected with vEBNA3A. CD8+ T cells (200 [▪]; 100 [░]; 50 []; or 25 [■]) were cultured with the indicated APCs in each well. Each bar represents the average number of spots in duplicate wells.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/4/10.1182_blood-2002-04-1240/3/h80433810006.jpeg?Expires=1722803103&Signature=x24HemNqLhBX51Y0WJay6MqvosYzvjm4QrsZzJ4uOJOrpFXgZaUsbPIi-acJvN4XtcbDPEJIG9Tj4Aou83o3T~lupaFF9-X4rbVKOz2zTUjkNB0LzQ7MnFc5CVxv8idCJrBnKO06cZpg7UtOpY8pIte4Fg6KV-byo3sBCgsXLizUaW76C3cKm06c1nQpd5xn3V8erKCRTmeRfMGO0voARPI0Nrj4SandWLJDifA2ZcOTQzchry7v~8CRCpS7Y8JsBVWlZBMzOF7RjfA8Fw2OWifYQxQTAAmI~7~NOdnZjcayMkQSLW~ntTC9adu06vc0n0Y60vw8w8ZaFxmoMDEYTQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Results of ELISPOT assays using CD8+ T-cell clones.
IYV-CTL is a clone specific for peptide no. 11, IYVLVMLVL; RYS-CTL, a clone specific for peptide RYSIFFDYM. LCL1 and 2 were established from PBMCs of HLA A24–positive donors, and LCL3 and 4 from HLA A24–negative donors. Abbreviations used for describing the stimulators are as follows: IYV, T2-A24 cells pulsed with peptide IYVLVMLVL; RYS, T2-A24 cells pulsed with peptide RYSIFFDYM; vL2, cells infected with vLMP2; vE3A, cells infected with vEBNA3A. CD8+ T cells (200 [▪]; 100 [░]; 50 [
]; or 25 [■]) were cultured with the indicated APCs in each well. Each bar represents the average number of spots in duplicate wells.
