Fig. 2.
Fig. 2. Down-regulation of TCR after stimulation with cognate peptides demonstrated by decreased levels of tetramer staining. / Before staining with each MHC/peptide tetramer (eg, Tet-11 means one incorporating peptide no. 11), aliquots of EBV-specific CD8+ T cells were incubated with T2-A24 cells in the presence of the same peptide used for making the indicated tetramer (eg, p11 for Tet-11, top row) or control HLA A24–binding peptide (eg, p13 for Tet-11, bottom row). After a 16-hour incubation, the cells were stained with phycoerythrin (PE)–conjugated HLA A*2402-tetramers and TRI-COLOR–labeled anti-CD8 mAb and analyzed by FACSCalibur. Numbers in the top right quadrants represent the percentages of tetramer-positive cells in the total CD8high cells.

Down-regulation of TCR after stimulation with cognate peptides demonstrated by decreased levels of tetramer staining.

Before staining with each MHC/peptide tetramer (eg, Tet-11 means one incorporating peptide no. 11), aliquots of EBV-specific CD8+ T cells were incubated with T2-A24 cells in the presence of the same peptide used for making the indicated tetramer (eg, p11 for Tet-11, top row) or control HLA A24–binding peptide (eg, p13 for Tet-11, bottom row). After a 16-hour incubation, the cells were stained with phycoerythrin (PE)–conjugated HLA A*2402-tetramers and TRI-COLOR–labeled anti-CD8 mAb and analyzed by FACSCalibur. Numbers in the top right quadrants represent the percentages of tetramer-positive cells in the total CD8high cells.

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