Fig. 5.
Fig. 5. PolyA Northern blot analysis of. / Evi3 and Ebfaz expression and Ebfaz RT-PCR. (A) Upper panel shows the results from Northern hybridization with a 3′ UTR Evi3probe. Middle panel shows the results from hybridization with a 3′ UTREbfaz probe. The lower panel shows Gapdexpression. B indicates brain; H, heart; K, kidney; Li, liver; Lu, lung; M, muscle; Sk, skin; Si, small intestine; Sp, spleen; St, stomach; Te, testis; and Th, thymus. (B) Upper panel shows RT-PCR analysis of Ebfaz expression. One primer was inEbfaz exon 3, and the other was at the transition between exons 4 and 5. F Brain indicates fetal brain; FL, fetal liver; BM, bone marrow; Pro-B, IgM−IgD−CD43+B220+cells; Pre-B, IgM−IgD−CD43−B220+cells; Imat B (immature B), IgM+IgD− cells; and Mat B (mature B), IgM+IgD+ cells. Lower panel shows the result from using Hprt control primers.

PolyA Northern blot analysis of

Evi3 and Ebfaz expression and Ebfaz RT-PCR. (A) Upper panel shows the results from Northern hybridization with a 3′ UTR Evi3probe. Middle panel shows the results from hybridization with a 3′ UTREbfaz probe. The lower panel shows Gapdexpression. B indicates brain; H, heart; K, kidney; Li, liver; Lu, lung; M, muscle; Sk, skin; Si, small intestine; Sp, spleen; St, stomach; Te, testis; and Th, thymus. (B) Upper panel shows RT-PCR analysis of Ebfaz expression. One primer was inEbfaz exon 3, and the other was at the transition between exons 4 and 5. F Brain indicates fetal brain; FL, fetal liver; BM, bone marrow; Pro-B, IgM−IgD−CD43+B220+cells; Pre-B, IgM−IgD−CD43−B220+cells; Imat B (immature B), IgM+IgD− cells; and Mat B (mature B), IgM+IgD+ cells. Lower panel shows the result from using Hprt control primers.

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