The 3′ LTR-driven expression of

Evi3. (A) RT-PCR analysis of RNA from the 7 AKXD27 tumors. One primer is located in the 3′ LTR sequence, and the other in exon 4 of Evi3. Hprt primers were included as a control for reverse transcription (lower panel). (B) Part of the sequence from the amplification products in panel A, showing the transition between LTR (shading) and intron 1 (small letters) and/or exon 2 (boxed capital letters). The ATG start codon is in reverse boldface. The RT-PCR sequence is identical to the sequence cloned by IPCR except that the RT-PCR product lacks introns 2 and 3. (C) Genomic sequence data from 4 previously cloned Evi3 integrations, showing the LTR/intron/exon transitions.