Interaction of 2 individual domains of PfEMP1 expressed as recombinant GST-fusion proteins to human endothelial cells.

Binding of DBL1α (panels A-E,K-M) and CIDR1α (panels F-J) to nontreated HUVECs (panels A-B,F-G) or HLECs (panels K-L); heparinase III–treated HUVECs (0.2 IU/mL for 2 hours) (panels C,H); PECAM-1/CD31–blocked HUVECs (10 μg/mL anti–PECAM-1/CD31 antibody) (panels D,I); and soluble HS–treated (1 mg/mL) HUVECs (panels E,J) and HLECs (panel M). Binding was visualized by incubating the cells with a mouse anti-GST antibody following incubation with an FITC-conjugated antimouse antibody and analyzed by light microscopy (× 1000). Scale bar equals 15 μm.