Heparinase sensitivity in adhesion of infected erythrocytes to human endothelial cells.

Adhesion of infected erythrocytes to human endothelial cells is heparinase sensitive. (A-B) Adhesion of FCR3S1.2-infected erythrocytes to HUVECs (panel A) and HLECs (panel B) stained with Giemsa (“Materials and methods”). Original magnification × 1000. (C) HUVECs were treated with the enzymes neuraminidase (▵), chondroitinase ABC (○), hyaluronidase (●), or heparinase III (▪) for 2 hours before incubation with FCR3S1.2 for 1 hour at 37°C. All samples are compared with controls in which pRBCs alone were incubated with untreated HUVECs. All data are expressed as the mean of 3 independent experiments ± SD.