Selectin binding and HECA 452 antigen expression on neutrophils before and during fucose therapy.

Neutrophils purified from a healthy donor (top panels) or the LADII patient (days 0-84) were analyzed for P- or E-selectin binding or HECA 452 antigen expression by flow cytometry using recombinant human P-selectin IgG or murine E-selectin IgM chimeras or the HECA 452 antibody. Filled histograms represent control stainings performed in the presence of 5 mM EDTA or rat IgG, respectively. Patterns similar to those on day 84 were obtained up to day 232 of treatment.