In vitro restoration of fucosylated glycoconjugates after fucose supplementation of a LADII lymphoblastoid cell line derived from the patient.

Control (B7; left panel) or LADII-derived (DF1; right panel) EBV-transformed lymphoblastoid cells were kept in culture for 5 days in the presence (Fuc) or absence (Untr) of 10 mM L-fucose. Fucosylated glycoconjugates on the cell surfaces were detected with the AAL lectin. Filled histograms represent control samples in which staining was carried out in the presence of 100 mM L-fucose.