D9:E9 activation by native and Arg2150His I2144-T2161 peptides and rFVIII.

D9:E9 (0.5 × 10⁴ cells per well) was incubated with the FVIII-specific lymphoblastoid cell line BO2C11 (0.5 × 10⁴ cells per well) pulsed with native (○) and mutated (●) I2144-T2161 peptide or recombinant FVIII at the indicated concentrations: (A) BO2C11 pulsed with native and mutated I2144-T2161 peptides; (B) BO2C11 loaded with native and mutated rFVIII molecules. Results are indicated as mean ± SD of duplicates.