Vacc.polyLMP immunization generates strong CTL activity in vivo.

Vacc.polyLMP or Vacc.TK⁻-immunized mice were inoculated with CFSE-labeled uncoated and peptide-sensitized (YLL or YLQ) splenocytes at 21 days after immunization. The clearance of these cells was monitored 18 hours after inoculation by FACScalibur. Ratios in the top right corner of each plot represent the percentage of peptide-pulsed, high CFSE-labeled cells to the percentage of low CFSE-labeled uncoated splenocytes retrieved from inoculated mice. A ratio of 1 indicates little or no clearance of peptide-labeled cells. Figures are representative values from individual mouse from a group of 4 mice. Data are representative of 2 independent experiments.