Antiubiquitin antibodies restore reduced TNF-α–producing capacities in blood from trauma and sepsis patients.
(A-B) Effect of antiubiquitin antibodies on LPS-induced TNF-α secretion in blood of multiply-injured (A) and sepsis (B) patients. Whole blood was incubated with LPS (100 ng/mL) for 4 hours. AS indicates antiubiquitin AS diluted 1:102 and 1:10 in the cell cultures; UbP4D1 and Ub N19, diluted 1:103 in the cell cultures; volunteers, whole blood cultures from healthy donors; TNF production (%), percent of the TNF-α secretion in trauma (A) and sepsis (B) patients' whole blood incubated without antibodies. Values are mean ± SEM from 5 healthy donors, 5 trauma patients, and 5 sepsis patients. (C) Effect of antiubiquitin AS (1:10) on LPS-induced TNF-α secretion in blood of uninjured donor and trauma and sepsis patients. Individual values from panels A and B. Data are pg/mL TNF-α.
