Effect of exogenous ubiquitin on LPS-induced TNF-α, IL-6, and IL-8 secretion of human whole blood and PBMNCs.

Whole blood (▪) and PBMNC () cultures (in duplicates) from healthy donors (n = 3) were incubated for 4 hours and 24 hours with 0 to 1 μg/mL exogenous ubiquitin in the presence of 100 ng/mL LPS. Data represent means ± SEM. *P < .05 versus cultures without ubiquitin. TNF-α/IL-6/IL-8 production (% control) is percent of TNF-α/IL-6/IL-8 secretion in cultures without exogenous ubiquitin. (A) TNF-α secretion, 4 hours of incubation. (B) TNF-α secretion, 24 hours of incubation. (C) IL-6 secretion, 4 hours of incubation. (D) IL-6 secretion, 24 hours of incubation. (E) IL-8 secretion, 4 hours of incubation. (F) IL-8 secretion, 24 hours of incubation.