Secondary transplantation.

(A-B) Whole human BM cells obtained from each primary recipient mouse given an iBM transplant (n = 10) were transplanted to a secondary recipient mouse by intravenous injection (n = 5) (A) or iBM injection (n = 5) (B). Secondary recipient mouse BM cells were analyzed for the expressions of human CD45 at 6 weeks after transplantation. Each symbol represents 1 mouse, and bars indicate the average engraftment level in 3 independent experiments. (C) Representative FACS analysis of a NOD/SCID mouse that received a secondary transplant. Human CD45⁺ cells in BM were analyzed for the expression of CD19 (ii), CD33 (iii), and CD34 (iv). The relative frequencies of each population are indicated.