Representative FACS profile of human multilineage engraftment in a NOD/SCID recipient given transplants of Lin^−/lowCD34⁺CD38⁻ cells by iBM.

(A) At 8 weeks after transplantation of 500 Lin^−/lowCD34⁺CD38⁻ human CB cells, BM cells were removed from iBM-injected side tibia of a NOD/SCID mouse and analyzed for the presence of human CD45⁺ cells (i). Human lineage–specific mAbs were used to detect lymphoid CD45⁺CD19⁺ (iii), CD45⁺CD56⁺ (v), myeloid CD45⁺CD14⁺ and CD45⁺CD33⁺ (ii,iv), and immature CD34⁺CD38^−/low (vi,vii) progenitor cells in the marrow of engrafted NOD/SCID mice. (B) Distribution of human hematopoietic cells in iBM-injected NOD/SCID mice. Eight weeks after transplantation, BM cells (ii,iii), spleen cells (iv), and PB (v) of NOD/SCID mice were stained with antihuman CD45 mAbs and analyzed by flow cytometry. BM cells were collected separately from injected (ii) and noninjected (iii) tibiae. BM cells that were injected with irradiated CD34⁺CD38⁺ (used as carrier cells in these experiments) alone were used as negative control (i). The relative frequencies of each population are indicated. Representative FACS analysis of 5 independent experiments is shown.