Summary of human cell engraftment levels in the BM of NOD/SCID mice.

(A-B) NOD/SCID mice were given transplants of CB Lin^−/lowCD34⁺CD38⁻ cell fractions by iBM (●) (A) or intravenous (○) (B). Eight weeks after transplantation, BM cells obtained from NOD/SCID mice were analyzed by FACS and PCR. Numbers indicate the dose of Lin^−/lowCD34⁺CD38⁻ cells transplanted. Number “0” means mice were given transplants of 10⁴ irradiated CD34⁺CD38⁺ carrier cells alone. Each dot represents 1 mouse, and bars indicate the average levels of engraftment. The horizontal lines indicate threshold of positive engraftment. (C) Representative PCR analysis of individual NOD/SCID mice given transplants of CB Lin^−/lowCD34⁺CD38⁻ cells by iBM (lanes 1-5 indicate 100 cells injected, lanes 6-10 indicate 10 cells injected). DNA was extracted from the BM cells 8 weeks after transplantations and amplified using a human chromosome 17 α-satellite specific primer. Representative PCR analysis of 5 independent experiments is shown. M, size marker; P, 100% human DNA; Mo, 100% mouse DNA; N, distilled water (DW).