Western blot analysis of ICAM-4 in transfected COS-7 cells and culture supernatant.

COS-7 cells were transfected with a GFP ICAM-4 or a GFP ICAM-4S fusion construct. Whole cell lysates (A) and TCA-precipitated proteins from tissue culture supernatants (B) were analyzed by immunoblots of 7% acrylamide gels probed with anti-GFP. ICAM-4 (lane 1); ICAM-4S (lane 2).