IL-7Rα down-regulation by IL-7 requires metabolic activity.

Expression of IL-7Rα on CD3⁺CD4⁺ (A) and CD3⁺CD8⁺ (B) T cells following incubation of cynomolgus PBMCs with IL-7 (10 ng/mL) for 24 hours. (Thin solid line, paraformaldehyde-fixed cells; gray line, unfixed cells incubated at 4°C; thick black line, unfixed cells incubated at 37°C; dotted line, isotype control).