FISH analysis of Y-chromosome in the glomerular mesangial cells in male-to-male transplantation.

A glomerulus was stained with anti-GFP (red; panel A) and an FITC-labeled Y-chromosome paint probe (green; panel B). Panel C shows triple labeling of the same glomerulus with a combination of anti-GFP, FITC-labeled Y-chromosome paint probe, and bis-benzimide (blue; nuclear label). Panel D is a superimposition of panels A and B. Two of 3 cells (arrows) are donor-derived mesangial cells with only one Y-chromosome. One cell shown has one Y-chromosome and is of recipient origin. Original magnification, × 63.