Multilineage hematopoietic reconstitution by cells derived from a single EGFP⁺ cell.

Nucleated peripheral blood cells of a recipient mouse were analyzed using flow cytometry 2 months after transplantation. (A) Blood cells from a control Ly-5.1 mouse that did not receive a transplant were stained with isotype-matched immunoglobulin. (B-D) Reconstitution by EGFP⁺ cells of Gr-1⁺ and/or Mac-1⁺cells, B220⁺ cells, and Thy-1.2⁺ cells are shown, respectively. The percentages of the engrafting cells in the granulocyte/macrophage, B-cell, and T-cell lineages were 79%, 77%, and 15%, respectively.