Examples of methylation analysis of CpG islands at the promoter area of JunB gene.

The sequence shown corresponds to the −77 to −116 to the transcriptional start site of JunB gene. (A) Unmodified DNA with all the cytokines remains intact. (B) Modified DNA of an unmethylated sample with all the cytokines converted to thymidine. (C) Modified DNA of a methylated sample with all Cs in CpG dinucleotides remaining as C. (D) Modified DNA of a partially methylated sample with part of the Cs in CpG dinucleotides showing C/T heterozygosity. Blue indicates cytosine (C); black, guanine (G); red, thymidine (T); and green, adenosine (A).